| 1. | Then the pcr product was purified , ligated into pgem - t vector by ta cloning
筛选阳性克隆，测序，大量制备序列完全正确的质粒。 |
| 2. | The pcr product was cloned into pmd18 - t vector . the positive recombinant clone was identified by pcr and endonuclease digest
提取重组质粒经pcr鉴定和酶切鉴定后，对插入片段进行序列测定及分析。 |
| 3. | The product of pcr named vp6 is approximate 1 . 3kb in length . the vp6 gene was cloned into pmd18 - t vector and sequenced
将其插入克隆载体质粒pmd18 - t的ecorv酶切位点处，构建重组质粒pmd18 - t ? vp6 。 |
| 4. | Pgem - t vector system , jm109 competent cells , reverse transcription kit and in vitro translation kit were purchased from promega company
Pgem一t载体，感受态细菌jm109 ，反转录试剂盒和体外翻译试剂盒均购自promega公司。 |
| 5. | 2 . pcr products can be purified by ctab method , which removing the dntp and primers . the purified products can be used in the ligation reaction to pmd18 - t vector with high efficiency
2 、采用ctab进行pcr产物的纯化，以去除pcr产物中的引物和dntp ，所得产物可用于与t载体连接反应，以提高连接效率。 |
| 6. | 2 . using a pair of degenerate primers based on the conserved region , hmg - box , of human sry gene , eight different fragments were amplified from both female and male rana rugulosa wiegmann then cloned by using pmd18 - t vector and sequenced
2 、参照人sox基因设计了一对兼并引物，扩增了虎纹蛙的sox基因，并对扩增产物进行了克隆和测序。 |
| 7. | To device a primer pairs and amplify the full length pstvd by rt - pcr , the positive rna extraction from tuber sample was used as template . the product of rt - pcr was purified and connected to the plasmid pmd 18 - t vector
Cdna核酸斑点杂交反应( nash )检测pstvd方法准确、灵敏度高，一次检测样品数量多，且对于异地样品检测非常方便，是以往其它检测方法的有效补充。 |
| 8. | Two degenerate primers were designed and synthesized according to the highly conservative sequences among the known - glc genes . a cdna fragment of 208bp was amplified by rt - pcr , which was subsequently cloned into pmd18 - t vector for sequencing analysis
利用genbank中已经登录的其它植物中该酶基因的保守序列，设计一对简并引物，采用rt - pcr技术，从茶树扩增出208bp的cdna片段。 |
| 9. | The tmek2 and tmek2mut was amplified by pcr and then cloned to pmd 18 - t vector . to express the genes in plants , the coding region of tmek2 and tmek2mut were placed between the 35s promoter and nos terminator of pibl , respectively
载体构建的过程包括目的基因的pcr扩增、目的基因连接到pmd18 - t载体、目的基因连接到中间表达载体pib1 、目的基因及启动子、终止子连接到表达载体pbin19 。 |
| 10. | Designing a specific primer pair based on the sequence of the gene ubia in e . coli mc4100 , a dna fragment was amplified from the genomic dna of e . coli mc4100 by pcr and then was cloned into pucm - t vector . the cloned fragment was sequenced
根据e . colimc4100的ubia基因全碱基顺序，合理设计引物，以e . colimc4100基因组dna为模板进行pcr扩增，将pcr产物克隆于pucm - tvector后对其进行测序鉴定。 |
